VE-BEVS Transfer Vector Kit 20040 (pAcVE1, pAcVE.02 and pAcVE.03)

20040 (10 µg each)

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ParaTechs offers all three VE-BEVS Transfer Vectors as a kit (20010, 20020 and 20030) for retail. Each is a baculovirus transfer vector designed for high levels of recombinant gene expression under the control of the Autographa californica nucleopolyhedro virus (AcMNPV) polyhedrin promoter.

Enhanced recombinant protein production in insect cells when utilizing ParaTechs’ Vankyrin-Enhanced (VE) transfer vectors. Recombinant protein production is further enhanced when used in combination with our VE-insect cells.

  • Expression of gene of interest from polyhedron promoter
  • Co-expression of vankyrin gene from p10 promoter

pAcVE1 Transfer Vector (20010)

This baculovirus transfer vector integrates the co-expression of a vankyrin gene for increased protein production.

Click here to view/download the:

pAcVE.02 Transfer Vector (20020)

This baculovirus transfer vector integrates the co-expression of a vankyrin gene for increased protein production with:

  • honey bee melittin signal sequence for direct secretion and
  • N-terminal 8x His-tag for ease of purification

Click here to view/download the:

pAcVE.03 Transfer Vector (20030) 

This baculovirus transfer vector integrates the co-expression of a vankyrin gene for increased protein production with

  • honey bee melittin (HBM) signal for direct secretion and
  • optional C-terminal 6x His-tag for ease of purification

Click here to view/download the:

Documents

    Transfer Vector pAcVE1 (PDF)
    Transfer Vector pAcVE.02 and pAcVE.03 (PDF)
    pAcVE1 Instructions (PDF)
    pAcVE.02 Instructions (PDF)
    pAcVE.03 Instructions (PDF)
    VE-BEVS Licensing

    Contract service for recombinant virus construction is available upon request.

    Patent information:
    VE-BEVS Cell Line United States Patent 7,842,493 [PDF]
    VE-BEVS Transfer Vector United States Patent 7,629,160 [PDF]

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